Webinar on March 5th
Exploring Methodologies for Spatial Biology, from IF to IHC to multiplex IHC with SignalStar® Technology
Multiplex spatial biology enables the simultaneous detection of multiple protein targets, revealing cellular dynamics within their native spatial context and leading to a more comprehensive understanding of complex biological processes and systems.
In this seminar, we will provide a comprehensive overview of the basic immunodetection techniques necessary for spatial biology, namely Immunohistochemistry (IHC) and Immunofluorescence (IF).
You will learn how to leverage these techniques, building toward advanced spatial biology applications. Two distinct multiplex solutions will be presented:
- For low-plex Immunofluorescence (IF-F) panel design. We will introduce chimeric antibodies that overcome common challenges in host-species compatibility.
- Multiplex IHC with SignalStar Technology: This technology allows you to multiplex up to eight protein targets in precious FFPE samples. We will explain how low-abundance targets can be detected with SignalStar Technology with it’s unique signal amplification. This validated product includes an optimized protocol, enabling researchers to generate high-quality results in only two days using standard fluorescent microscopy equipment.
Presented by: Flora Guarnotta, MSc Field Application Specialist at CST
New Products this month from CST!
NG2/CSPG4 (F8X3M) Rabbit
Monoclonal Antibody #54851
IF-F: Confocal IF analysis of fixed mouse cerebellum labeled with #54851 (green), TMEM119 (E4B9S) Mouse Monoclonal Antibody #98778 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Proteoglycan Involved in Cell Scaffolding and Signaling
NG2 is a transmembrane chondroitin sulfate proteoglycan present on the cell surface. It promotes cell proliferation, migration, and metastasis through phosphorylation by PKCα and ERK. It is also expressed in vascular pericytes to regulate angiogenesis, and in the nervous system, it is involved in myelin repair as a marker of oligodendrocyte precursor cells. It is highly expressed in intractable cancers such as melanoma, glioblastoma, and triple-negative breast cancer, and is associated with treatment resistance and metastasis. Taking advantage of its unique expression pattern, NG2 is currently being clinically applied as a promising target for cancer immunotherapy, such as CAR-T cell therapy. #54851 is suitable for WB, IP, IF-F, IF-IC, FC-FP, and FC-L analysis using human, mouse, and rat samples.
BRD9 (F6F1S) Rabbit Monoclonal
Antibody #98202
ChIP: ChIP were performed with cross-linked chromatin from NIH/3T3 cells and either #98202 or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human RCN3 promoter primers, human ZFP948 intron 1 primers, and SimpleChIP® Mouse Intracisternal A-Particle (IAP) LTR Primers #85916. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin Remodeling Component
BRD9, a component of the non-canonical BAF complex, recognizes acetylated histones via its bromodomain and targets the complex to specific gene promoters and enhancers. BRD9 is particularly involved in maintaining stem cell pluripotency, cell differentiation, and regulating immune responses, regulating the expression of genes essential for cell fate determination. It has also been reported to be essential for tumor survival in synovial sarcoma and malignant rhabdoid tumors, which possess characteristic gene fusions. BRD9 is also involved in the proliferation of acute myeloid leukemia (AML), and in recent years, development of drugs targeting BRD9, such as PROTACs (targeted protein degradation inducers), has been progressing. #98202 is suitable for WB, IP, and ChIP using mouse and rat samples.
New Products This Month from NEB!
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