Check out our latest Research & Biotech promotions and events

Exclusive 30% Discount* on Monarch Nucleic Acid Purification kits and Luna (RT-)qPCR mixes’

Choose Monarch for uncompromising quality of your nucleic acid purification

Monarch nucleic acid purification kits provide fast and reliable isolation and purification of high quality DNA and RNA from a variety of sample types. Technologies used include best-in-class silica columns, silica‑coated mag beads, or glass‑beads for the isolation of high molecular weight DNA. The resulting nucleic acids are highly pure and ready for sequencing, cloning, PCR, and other enzymatic applications.

The Monarch nucleic acid purification portfolio can serve your needs, whether you are isolating nucleic acids from biological samples, cleaning up DNA and RNA from enzymatic reactions, extracting DNA fragments from gels, or purifying plasmids.

Monarch DNA and RNA kits use purposefully reduced‑plastic columns and bottles and come in compact packaging, providing environmental responsibility without compromising on performance.

Optimize your (RT-)qPCR with Luna

  • Convenient master mix and supermix formats and user-friendly protocols simplify reaction setup.
  • Non-interfering, visible tracking dye eliminates pipetting errors.
  • Formulated with a unique passive reference dye, compatible across a wide variety of thermal cyclers.
  • Luna WarmStart RT paired with Hot Start Taq enables room temperature setup and stability.
  • 4X option allows for more sample input, increasing sensitivity.
  • Available in a lyophilized format.
  • Offer valid from April 1st, 2026 to June 30th, 2026
  • Use promo code LUMO2026 when ordering
  • *30% discount on the catalogue price

Rethinking mRNA Workflows: From Manual Processes to Automated Precision

In this Virtual Lunch and Learn, professionals from EMBL (GeneCore), New England Biolabs, and SPT Labtech will outline the journey toward automating the NEBNext UltraExpress mRNA library preparation workflow on the SPT Labtech firefly+ platform. They will detail the individual steps involved in transforming the workflow, from initial manual testing on the bench to a fully automated solution.

Ferris JungAlicia He, and Jing Zhang will discuss key considerations when translating a manual protocol into an automated workflow, including liquid-handling constraints and strategies to maximize the capabilities of the instrument.

The session will also highlight challenging steps encountered during implementation, along with the approaches used to address them and ensure robust performance. Attendees will gain insight into how to systematically convert a manual library preparation workflow into an automated solution, improving consistency, reproducibility, and scalability in laboratory environments.

What you’ll learn:

  • Approaches to improving reproducibility and reducing hands-on time in mRNA library preparation
  • Key steps and considerations for automating the NEBNext UltraExpress mRNA workflow
  • Common challenges in method transfer and how to overcome them on the firefly+ platform

Tuesday, June 30th. 8 AM PT | 11 AM ET | 5 PM CEST

New Products This Month

Non-phospho NFAT1 (Ser217/Ser221) (F4L8I) Rabbit Monoclonal Antibody #38950

Panel of four flow cytometry dot plots comparing untreated (left) and treated (right) samples. Top row plots show CD3-PE Conjugate versus Non-phospho NFAT1 (Ser217/Ser221); bottom row plots show Rabbit IgG isotype control versus CD3-PE Conjugate. Used to compare NFAT1 phosphorylation between conditions across isotype controls.

FC-FP: FC analysis of fixed/permeabilized human peripheral blood mononuclear cells, untreated (left column) or treated with Rapid-Act T Cell Activation Kit (Human, Anti-CD3/CD28) #88179 (15 min; right column), using #38950 or concentration-matched Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 (bottom row), co-stained with CD3 (UCHT1) Mouse Monoclonal Antibody (PE Conjugate) #46233. Anti-rabbit IgG (H+L), F(ab’) Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody

Mechanisms of regulatory activity of the NFAT transcription factor family and calcium signaling.

The NFAT family (NFAT1–4) are transcription factors possessing a Rel homologous domain, and they work in conjunction with AP-1 to regulate the transcription of immune response-related genes. Calcium signaling is central to their activity regulation. NFAT1, highly phosphorylated in the quiescent phase, is dephosphorylated by activated calcineurin in response to increased intracellular calcium concentration and translocates to the nucleus. Conversely, rephosphorylation by GSK-3 and CK1 in response to decreased calcium concentration terminates the signal. Immunosuppressants such as cyclosporine A and FK506 are known to inhibit calcineurin activity, thereby preventing NFAT nuclear translocation. This pathway is a crucial molecular basis for T cell activation. #38950 is suitable for WB, IP, and FC-FP using human samples.

KLF2 (F4Q2I) Rabbit Monoclonal
Antibody #61741

 

Two-panel fluorescence microscopy image: left panel shows green-labeled cells, right panel shows red and blue-stained cells (overlay of markers).

IF-F: Confocal IF analysis of fixed frozen mouse lung labeled with #61741 (green). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate) #19581 (gray pseudocolor), CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #34624 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).

Mechanisms of regulatory activity of the NFAT transcription
factor family and calcium signaling. 

Krüppel-like factors (KLFs) are a group of zinc finger-type transcriptional regulators homologous to Krüppel, a somite formation factor in Drosophila. Currently, 17 types have been identified in mammals, regulating a wide range of life phenomena including development, differentiation, proliferation, and stress response. Dysfunction of these KLF family members is involved in the pathogenesis of various diseases, such as metabolic disorders and cancer. KLF2, in particular, is a key factor in the differentiation and maintenance of function of immune cells and vascular endothelial cells. KLF2 exerts anti-inflammatory effects by suppressing the expression of inflammatory genes via the NF-κB pathway, playing a crucial role in regulating immune responses and maintaining tissue homeostasis. #61741 is suitable for WB, IHC-P, and IF-F using mouse samples.

New Products This Month from NEB!

  • Get precise results with Q5’s high fidelity (~280x Taq)
  • Reduce turnaround time through fast cycling conditions, with extension times of 5-15 seconds/kb
  • Optimized formulation enables robust, reliable amplification of diverse targets (from high AT or high GC) up to 20 kb
  • Improve reaction specificity with a hot start aptamer that allows a convenient room temperature setup
  • Compatible with a 62°C universal annealing temperature protocol. Learn more in our application note
  • Also available: Q5 standalone polymerase (NEB #M0491, NEB #M0493, NEB #M0515) and master mix formats (NEB #M0492, NEB #M0494, NEB #M0578, NEB #M0580)

Latest Blog

Choosing the right human preadipocyte cell model for reliable metabolic assays

Human preadipocyte cell models are widely used to study adipogenesis, obesity, insulin resistance, and metabolic disease mechanisms in vitro. However, assay outcomes can vary depending on donor background, adipose depot origin, differentiation capacity, and culture conditions. Selecting the appropriate human preadipocyte model is therefore essential for improving reproducibility, translational relevance, and metabolic assay consistency.

Connect With A Product Specialist

Have you got question? We’d love to hear from you… 

    We’re here for you.

    If you have a question or query relating to specialised equipment or products fill out a contact form. Our Industrial Product Specialists are standing by, waiting to speak with you.